Eight of twenty-five genes fulfilled the criteria for protein-level validation but due to unavailability of flow cytometry antibodies for STAB1 and AQP9, only CD300b, TLR4, CCR2, THBD, FZD1, and WLS could be validated on 11 independent donors from the same TB endemic cohort (which were not included in the sequencing data) (Figure S4B). This evidence concerns the gene TLR4 and tuberculosis.