THBS1 and liver failure: To validate our findings regarding the potential role of THBS1 in liver failure and its ability to predict disease severity and outcome, we further assessed the influence of THBS1 profile using an ALF model generated in both hepatocyte-specific THBS1KO and WT mice, as illustrated in Fig. 5A. The D-gal/LPS combination could induce ALF in animals, closely resembling the clinical syndrome, mimicking the systemic inflammatory responses and leading to multiorgan failure [28, 29].