Proximity-based interactions between Tregs and both monocytes (p = 0.009) and CD8<sup>+</sup> T cells (p = 0.009) were more frequently found in non-responding patients, while macrophages were more frequently located in proximity to HLADR<sup>+</sup> tumour cells (p = 0.01) within responding patients. Here, CD8A is linked to neoplasm.