In clinical application, medical staff can add the corresponding scores of various risk factors (MALAT1, NLRP3, age, SBP, smoking) in the nomogram, and get the risk of lower limb atherosclerosis in T2DM patients according to the total score, which is helpful for clinical evaluation of the condition according to the individual condition of patients. The gene discussed is MALAT1; the disease is type 2 diabetes mellitus.