In this study, we confirmed the differential expression of lncRNA MALAT1 and NLRP3 in T2DM patients with LEAD and T2DM patients alone and found that lncRNA MALAT1 can be used as a potential biomarker for lower limb atherosclerosis in patients with T2DM, and revealed the correlation between lncRNA MALAT1 and NLRP3. This evidence concerns the gene MALAT1 and type 2 diabetes mellitus.