We then used a Transwell assay to assess the effect of TMEM176B silencing on GC cell migration/invasion and found that migration and invasion ability decreased significantly (Fig. 3A–D).We then used flow cytometry to investigate whether TMEM176B knockdown affects GC cell apoptosis and found that the proliferation ability of gastric cancer cells was significantly decreased (Fig. 3E–F). The gene discussed is TMEM176B; the disease is gastric cancer.