Gal3 has been reported to support stemness in ovarian cancer by activating the Notch1 signaling pathway [35–37], therefore we assessed the levels of intact and cleaved Notch1 (Notch1 intracellular domain (NICD)) in the ARK1 and 2 Gal3-CTRL and KO cells by immunoblotting. The gene discussed is NOTCH1; the disease is ovarian cancer.