As shown in Fig. 9, significant relationships were observed between ANKRD1 and immune checkpoint genes, such as including CD200R1, CD47, cytotoxic T lymphocyte associated protein 4 (CTLA4), herpes virus entry mediator A (HVEM), programmed death-1 (PD-1), PD-2, TIGIT, and TIM-3, in most cancers except ACC, CESC, DLBC, KIRC, UCEC, and UCS. The gene discussed is CTLA4; the disease is cancer.