In this study, we performed a systematic pan-cancer (including 33 different types of cancer) analysis to explore the expression profile, diagnostic value, prognostic value, microRNA (miRNA) regulation, drug sensitivity of ANKRD1, and the potential relationship between ANKRD1 expression and tumor stage, DNA methylation level, mutation status, immunological function, and enriched pathways. Here, ANKRD1 is linked to neoplasm.