Furthermore, we found that the DNA methylation level of ANKRD1 was positively related to ANKRD1 expression in BLCA, COAD, KIRC, LIHC, and TGCT, but negatively associated with ANKRD1 expression in BRCA, HNSC, MESO, prostate adenocarcinoma (PRAD), and sarcoma (SARC) (Fig. 4B). Here, ANKRD1 is linked to sarcoma.