Here, we found a significant decrease in the DNA methylation level of ANKRD1 in BLCA, BRCA, CESC, CHOL, COAD, HNSC, KIRC, KIRP, LIHC, LUAD, LUSC, OV, PAAD, pheochromocytoma and paraganglioma (PCPG), READ, THCA, and UCEC based on the TCGA database (Fig. 4A). This evidence concerns the gene ANKRD1 and hereditary pheochromocytoma-paraganglioma.