Previous research suggests that the expression of NOP2 primarily relies on the m5C methylation level, as it can promote cancer cell proliferation through m5C-dependent inhibition of Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B, p27Kip1) (Mei et al., 2020; Yang et al., 2023). The gene discussed is NOP2; the disease is cancer.