We used the HPA database to analyze the immunohistochemical staining results of Gasdermins protein in liver cancer tissues and normal liver tissues, the protein levels of GSDMB, GSDMD and GSDME staining concentrations were found to be increased in LIHC tissues compared with normal tissues, while GSDMA, GSDMC levels did not change (Figure 3B).Based on the above bioinformatics analysis, we further evaluated the expression of GSDME protein in LIHC, and Western blot detected GSDME in LO2 cell line (normal liver cells) and 2 human liver cancer cell lines (Hep-G2 and SK-Hep-1). The gene discussed is GSDME; the disease is liver cancer.