Here, we discovered that TRIM67 influences the malignant behaviour of tumour cells by promoting the proliferation, migration, and invasion of NSCLC cells via the Notch pathway and the expression of downstream proteins, including RhoA, RhoC, MMP-9, P21, c-Myc, and epithelial-mesenchymal transition-related markers 25. This evidence concerns the gene RHOA and non-small cell lung carcinoma.