In addition, we analyzed the correlation between PGAM1 and methylation-related genes, and the results showed that PGAM1 had significant correlations with methyltransferases such as DNMT1, DNMT3A and DNMT3B as well as methylation modifications such as m1A, m5C and m6A across various cancers (Supplementary Figure 3). This evidence concerns the gene DNMT3B and cancer.