ELAVL4 and pancreatic insulinoma: To investigate whether cellular zinc level could affect HuD expression in pancreatic β cells, HuD expression in mouse insulinoma βTC6 cells maintained in 25 mM of glucose/DMEM was analyzed after modulating intracellular zinc concentration by depletion (with a zinc-chelator TPEN), supplementation (with a nutrient supplement zinc sulfate (ZnSO4)), or knockdown of ZIP8.