Besides, LASSO regression demonstrated female age, female BMI, number of spontaneous abortions and female age (years), type of infertility, male age (years), female BMI(kg/m2), Gn days, MII oocytes, number of good quality embryos, number of available embryos, LH (IU/L) on hCG day, tubal factor, number of spontaneous abortions, number of induced abortions were potential independent risk factors for miscarriage (Supplementary Figure 1A), when λ was set at the minimum criteria lambda.1se (the left line) (Supplementary Figure 1B). Here, PLOD1 is linked to Miscarriage.