Considering that our previous study has uncovered that ROCK1 protein is more significant than ROCK2 protein in inducing cecal ligation and puncture-induced cerebral injury and cognitive impairment [22], ROCK1 was knocked down here and ROCK1 interference was noticed to enhance the viability, restore the mitochondrial membrane potential, reduce the cytotoxicity and apoptosis, down-regulate P-PTEN expression and up-regulate P-Akt, P-mTOR expression in Glu-treated HT22 cells. This evidence concerns the gene ROCK2 and Cognitive impairment.