Given that TRRUST analysis implicated RELA (p65) as a key transcription factor influenced by TP53-GOF mutation and osimertinib treatment in EGFR-mutated NSCLC cells, we examined the binding of p65 to the promoter region of the TNF gene by ChIP-qPCR analysis. The gene discussed is RELA; the disease is non-small cell lung carcinoma.