IFNG and Sepsis: To further understand the effect of PRDX3 on macrophage polarization during sepsis, we overexpressed PRDX3 in RAW 264.7 cells with lentivirus carrying PRDX3 cDNA (Adv-PRDX3) (Fig. 5A), and incubated the stable overexpression cells in medium with both LPS and IFN-γ.