Moreover, the expression of key proteins involved in cuproptosis, such as FDX1 and DLAT, is further upregulated in ICC cells with SLC39A4 knockdown, providing additional evidence that SLC39A4 knockdown can influence various cellular processes, including proliferation, apoptosis, and migration, through the cuproptosis pathway. Here, SLC39A4 is linked to intrahepatic cholangiocarcinoma.