For TIME, silencing PinX1 combined with IR significantly increased the percentage of CD8+ T cells (14.3 ± 0.71 IR vs 20.72 ± 1.16 shPinX1 + IR, % of CD3+ cells, p = 0.003, Fig. 3F, G) and Granzyme B+(GzmB) cytotoxic CD8+ T cells (35.12 IR ± 10.54 vs 53.80 ± 6.06 shPinX1 + IR, p = 0.001, Fig. 3J, K) compared to the IR group, as well as markedly decreased the percentage of CD8+ PD-1+ Tim3+ exhausted CD8+T cells in tumor tissues(37.40 ± 3.69 IR vs 20.08 ± 10.58 shPinX1 + IR, p = 0.012, Fig. 3H, I). The gene discussed is GZMB; the disease is neoplasm.