On the basis of this finding, we confirmed that miR-101-3p reexpression leads to single DNA-strand breaks (sDSB) as well as double DNA-strand breaks (dDSB), as validated by significantly increased phosphorylation of H2AX, increased cleaved PARP, and reduced PARP expression, as well as positive TUNEL staining in melanoma cells. This evidence concerns the gene H2AX and melanoma.