However, in HPV-negative HNSCC patients, LAYN was positively correlated with infiltrating B cells (P < 0.01), CD4 + T cells (P < 0.001), macrophages (P < 0.001), neutrophils (P < 0.05), and dendritic cells (P < 0.001) in tumor tissues. The gene discussed is CD4; the disease is head and neck squamous cell carcinoma.