Univariate analysis showed that the number of metastatic sites (> 2/ ≤ 2), the diameter of intrahepatic tumor (> 30/ ≤ 30 mm), LDH level (> 300 U/L/ ≤ 300 U/L), pre-treatment neutrophil-to-lymphocyte ratio (NLR > 2.8/ ≤ 2.8), CD4 + CD25 + cell level (> 23%/ ≤ 23%), CD16 + CD56 + cell level (> 20%/ ≤ 20%), and diameter of the ablated lesion (> 30/ ≤ 30 mm) were significantly correlated with the OS of patients (Fig. 2; Table 3). The gene discussed is NCAM1; the disease is neoplasm.