To further investigate whether GSDME cleavage in keratinocytes can be induced when keratinocytes are exposed to stimulation of psoriasis-like immune environment, HaCaT cells, an immortal human keratinocyte line, were exposed to a mixture of TNF-α, IL-17A, IL-22, Oncostatin M (OSM), IL1-α (Mixture 5, M5). Here, IL22 is linked to psoriasis.