Furthermore, MTT assay, colony formation assay, transwell assay and western blot assay results exhibited that overexpression of CDK1 could partially restore the phenotypes of GBM cell proliferation and invasion inhibition caused by OTUD4 knockdown, and the expressions of cell cycle and EMT-related proteins also changed correspondingly (Fig. 6E–H). This evidence concerns the gene OTUD4 and glioblastoma.