In conclusion, this study demonstrated that OTUD4 is an oncogene with prognostic significance in GBM, revealed a new mechanism by which OTUD4 directly deubiquitinates or indirectly regulates CDK1, and identified specific interaction regions and key amino acid residues of ubiquitin and CDK1(Fig. 7G). The gene discussed is OTUD4; the disease is glioblastoma.