Confocal analysis of HNSCC cells also showed that the nuclear expression of β-catenin decreased, while the cytosolic β-catenin remained unchanged in TRIB3 silencing cells compare to control cells, moreover, TCF4 mainly expressed in nuclear and its expression level remains almost unchanged in control or TRIB3 silencing cells (Fig. S4a). The gene discussed is TRIB3; the disease is head and neck squamous cell carcinoma.