MYOM2 and POEMS syndrome: Since the serum M protein amount in POEMS syndrome is not very high (1–2 g/dL), the positive rate of serum protein electrophoresis is about 75% and about one-quarter of patients with POEMS syndrome are negative for serum M protein, which requires more sensitive serum immunofixation electrophoresis to determine the presence of M protein.[11] Alfonso first reported immunofixation electrophoresis technology in 1964, which included the agar gel protein electrophoresis and immunoprecipitation operations and had the benefits of a short cycle, high sensitivity, and high resolution.