To further unearth the enzymes related to the regulation of ACLY acetylation level, acetyltransferase (PCAF) and deacetylase (HDAC1 and SIRT2) were selected for further study, we found that ACLY had significant interaction with PCAF and SIRT2 in ESCC cells, but not HDAC1. This evidence concerns the gene HDAC1 and esophageal squamous cell carcinoma.