To explore whether ACLY is acetylated in ESCC cells, Co‐IP was used to detect the acetylation of ACLY in Eca109 and KYSE150 cells by pan‐acetylated antibody (AC) as Co‐IP antibody for immunoprecipitation, and then Western blot was used to detect the expression of ACLY in protein complex. The gene discussed is ACLY; the disease is esophageal squamous cell carcinoma.