To further explore the enzymes involved in ACLY acetylation modification in ESCC cells, ACLY antibody was used as Co‐IP antibody for immunoprecipitation, and the expressions of acetyltransferases PCAF and deacetylases (HDAC1 and SIRT2) in the complex were detected by Western blot. This evidence concerns the gene ACLY and esophageal squamous cell carcinoma.