GLS2 and infection: To understand how S. aureus significantly increased severity and prolonged the duration of infected ischemic pressure wounds in diabetic mice, we analyzed transcription of genes encoding staphylococcal cytotoxins (hla, hlgA, hlgB, hlgC, lukD, lukE, lukF, lukG, lukH, and lukS) and TCRS using quantitative real-time PCR (qRT-PCR) on infected tissue homogenates at day 7 post-infection.