Since we identified significant improvement in survival for the cluster 2 signature (dendritic cells) and a trend towards significant detriment to survival for the cluster 4 signature (tissue resident/epithelial‐like macrophages) in the TCGA‐PAAD dataset, we performed fluorescent immunohistochemistry to qualitatively identify co‐staining of signature genes that may be important for the function of these cells, SEMA4A and TRIM29, respectively, with known myeloid markers. The gene discussed is SEMA4A; the disease is pancreatic adenocarcinoma.