In the second step, among these 18 biomarkers, we observed IGF-1 exhibiting a significant mediating role between HMGCR inhibition and hyperthyroidism risk (mediation proportion: 2.108%; 95% CI 0.173% to 4.389%; P = 0.038) (Figure 6, Supplementary Table S15). The gene discussed is IGF1; the disease is hyperthyroidism.