Finally, the detection of nude mice with ESCC xenografts in situ divided into four groups including lenti-NC, lenti-CST1, lenti-CST1 + rotenone and lenti-CST1 + PD98059 groups demonstrated the potential of CST1 in ESCC metastasis by regulating of OXPHOS and MEK/ERK pathways in vivo. Here, MAP2K7 is linked to esophageal squamous cell carcinoma.