To further verify the effect of CST1 on the metastasis of ESCC cells in vivo, we generated nude mice with ESCC xenografts in situ by injecting the ESCC cell line KYSE410 with CST1 overexpression into the outer muscle layer of the distal esophagus in the upper part of the gastric cardia.Then, nude mice were divided into four groups including the lenti-NC group, lenti-CST1 group, lenti-CST1 + rotenone group, and lenti-CST1 + PD98059 group, with four nude mice in each group. The gene discussed is CST1; the disease is esophageal squamous cell carcinoma.