To verify whether CST1 affects ESCC cell metabolism, we first defined the energy metabolism of six common ESCC cell lines with mitochondrial ATP/total ATP ratios ranging from 47.4 to 56.5% and mitoATP/glycoATP ratios ranging from 0.9:1 to 1.3:1 (Fig. 3A), suggesting that the main energy supply of ESCC cells is not derived from glycolysis. The gene discussed is CST1; the disease is esophageal squamous cell carcinoma.