We also assessed the effect of NSUN2 on E2F1 mRNA stability and found that NSUN2 depletion reduced the half-life of E2F1 mRNA in ovarian cancer cells (Fig. 4c, Supplementary Fig. 5a), suggesting that NSUN2 promoted m5C modification of E2F1 mRNA and facilitated its stability. Here, NSUN2 is linked to ovarian cancer.