To examine whether the inhibition of BLCA cell proliferation and migration following Parkin overexpression was caused by ROS, we added 10 mM NAC (N-Acetyl-L-cysteine, a ROS inhibitor) or 1 mM H2O2 solution to the empty vector- and Parkin-overexpressing cells. Here, PRKN is linked to bladder transitional cell carcinoma.