We established a representative orthotropic lung cancer model in female mice by i.v. administering Lewis lung carcinoma cell lines expressing GFP (LLC-GFP) and evaluated in vivo antiangiogenic cancer therapy efficacy of LNP-CAD9 co-encapsulating Cas9 mRNA/VEGFR2 single guide RNA (sgRNA) (Fig. 5a). The gene discussed is KDR; the disease is lung carcinoma.