Additionally, in NAFLD, the PPARGC1A rs8192678 risk A allele is associated with an increased risk, even after control for BMI and other confounding factors.639 Nevertheless, the Gly482Ser polymorphism of the PGC-1α gene is not associated with the metabolic syndrome in Danish Caucasian subjects.640 Interestingly, Huang et al. utilized engineered allele substitution at PPARGC1A rs8192678 to obtain homozygous AA, GG and heterozygous G/A isogenic cell populations. This evidence concerns the gene PPARGC1A and metabolic dysfunction-associated steatotic liver disease.