ADAM10 and autism: To confirm the autism-like behaviors was due to the impaired α-cleavage of CNTNAP2 at ADAM10 site, the adeno-associated virus (AAV) vector expressing CNTNAP2-C79 protein (AAV-C79) and AAV-EGFP (as the control) were generated and injected into the medial prefrontal cortex (mPFC) of the mutant (Cntnap2-I1254T) and CNTNAP2-KO mice (Cntnap2−/−) at the age of 3 weeks (Supplementary Fig. 6a).