In HepG2 and Huh7 hepatoma cell lines stably overexpressing ChREBP, the blockade of ChREBP-induced PI3K/AKT signaling by MK-2206 also significantly impaired the capacity of ChREBP to increase cell proliferation in vitro (Fig. 3e–g and Supplementary Fig. 4e–g). This evidence concerns the gene AKT1 and hepatocellular carcinoma.