As expected, LLC tumors grown in FIH+/+ mice have foci of infiltrating inflammatory cells including F4/80-positive macrophages, FOXP3-positive regulatory T cells (Treg), CD4 and CD8 T cells (an example of LLC tumor grown in FIH+/+ is shown in SI Appendix, Fig. S3A), confirming that the syngeneic LLC subcutaneous tumor model is suitable for studying the role of FIH on TME and tumorigenesis. This evidence concerns the gene FOXP3 and neoplasm.