As an NLRP3 alkylator, Bot-4-one enhances the ubiquitination of NLRP3 contributing to the suppression of NLRP3 inflammasome in bone marrow derived-macrophage (BMDMs) and monosodium urate-induced peritonitis mouse model (Shim et al. 2017). Here, NLRP3 is linked to peritonitis.