It is worth mentioning that the compounds enriched in the insulin resistance pathway that were mainly upregulated were diradylglycerols and uridine diphosphate-N-acetylglucosamine in the MCD group, indicating that MCD-induced NAFLD in mice led to easier glucose absorption by regulating PDK1/GLUT4 signal transduction. This evidence concerns the gene PDK1 and metabolic dysfunction-associated steatotic liver disease.