As a hallmark of the radiation response is the phosphorylation of histone H2A.X (γH2A.X) at the site of double-strand breaks, we used γH2A.X immunostaining to evaluate the accuracy of targeted RT in autochthonous pancreatic tumours localised using either iohexol or Fenestra LC contrast. Here, H2AX is linked to pancreatic neoplasm.