NLRP3 is known to play a central role in inflammasome activation and pyroptosis during alcohol-related liver disease (de Carvalho Ribeiro and Szabo, 2022; Brahadeeswaran et al., 2023), nevertheless we cannot exclude contribution of additional pattern-recognition receptors in liver and/or brain of our alcohol-induced ACLF model. This evidence concerns the gene NLRP3 and liver disorder.