In this study, Cirbp is identified to be a direct target of thermomiR-377-3p in NPC cells, while thermomiR-377-3p improves the sensitivity of NPC cells and cancer stem-like cells to hyperthermia by directly suppressing Cirbp expression, suggesting that thermomiR-377-3p is a promising therapeutic targets of hyperthermia for NPC. The gene discussed is CIRBP; the disease is nasopharyngeal carcinoma.