Figure 4C indicated that the expression of a great number of the member of heat shock protein family, such as HSP90AA1, HSP90AA2P, HSP90AA4P, HSP90AB1, HSP90B1, HSPA13, HSPA4, HSPA4L, HSPA8, HSPA9P1, HSPB11, HSPD1, HSPD1P1, HSPE1, HSPE1-MOB4 and HSPE1P2, was significantly up-regulated in hyperthermia-treated NPC cells, while the decreased expression of Cirbp was observed in heat-treated cells. Here, HSPA4 is linked to nasopharyngeal carcinoma.