Our results clearly demonstrated that Cirbp knockdown significantly repressed ATM-Chk2 and ATR-Chk1 pathways after hyperthermia, and consequently attenuated DNA damage repair ability of cancer cells and seriously impaired cancer cell survival, ultimately enhancing thermosensitivity in cancer cells and cancer stem-like cells, and tumor growth inhibition (Fig. 12). Here, ATR is linked to neoplasm.