NT-proBNP holds significance in predicting heart failure prevalence, mortality, and prognosis [45], given that NT-proBNP is not an enkephalin substrate, this study opted for NT-proBNP analysis, excluding enkephalin degradation and providing a more accurate reflection of changes in ventricular wall pressure after sacubitril/valsartan treatment. The gene discussed is NPPB; the disease is heart failure.