We noted that CMs from RUNX1 over-expressed CRC cells (HCT116 or RKO) significantly induce M2 polarization of THP-1 compared with control groups, which showed an increase in the proportion of CD68+CD206+ macrophage and CD68+CD200R+ macrophage (all P < 0.01, Fig. 3C). The gene discussed is RUNX1; the disease is colorectal carcinoma.