Importantly, the RUNX1 nuclear aggregation of CRC cells induced by M2 macrophages derived PDGF-BB was observed by confocal laser scanning, as shown in Fig. 6I and J. With the treatment of 20 ng/ml PDGF-BB, the protein expression levels of c-myc, LEF1 and cyclinD1 in HCT116 and SW480 cells were significantly up-regulated, indicating the activation of Wnt/β-catenin signaling pathway (Fig. 6K, Supplementary Fig. 3J). The gene discussed is MYC; the disease is colorectal carcinoma.