In a HCT116-THP-1 co-culture model, numbers of CD68+CD206+CD200R+ triple positive macrophages in the HCT116 RUNX1OE/THP-1 co-culture group significantly increased compared to the control group, indicating that RUNX1 overexpression in CRC cells skew the macrophage polarization toward M2 macrophage (P < 0.01, Supplementary Fig. 2F). Here, MRC1 is linked to colorectal carcinoma.