To screen potential LASS2-interacting factors and regulatory signalling pathways, we performed co-IP-coupled LC–MS analysis of the human papillary thyroid carcinoma cell line BCPAP, the human triple-negative breast carcinoma cell line MDA-MB-231, and the mouse HCC cell line Hepa1-6 cells. The gene discussed is CERS2; the disease is triple-negative breast carcinoma.