The clonogenicity assay, mimicking stemness properties of cancer cells, further elucidated the role of Jarid2. The ability of cells to reconstitute the culture, particularly under hypoxia or with exogenous Tfcp2l1 expression, was partially suppressed when Jarid2 was silenced via siRNA (Supplementary Fig. S9B). The gene discussed is TFCP2L1; the disease is cancer.