Similar to what we observed with NCRM-1 hiPSCs, the phMNENRICHED condition resulted in a roughly 3-fold enrichment in SCIP+ phMNs derived from ALS-patient and isogenic controls of both CS29 and CS52 lines (Supplementary Fig. 5f), demonstrating the applicability of this method to multiple hiPSC lines. The gene discussed is POU3F1; the disease is amyotrophic lateral sclerosis.