LPCAT2 and colon carcinoma: To assess whether colibactin may directly trigger lipid overload, the human colon carcinoma HCT116 cells were infected with CoPEC strain (11G5) isolated from a patient CRC or a mutant strain that does not produce Colibactin (11G5∆clbQ).19 In agreement with our spatial metabolomic analysis, CoPEC infection leads to lipid droplet accumulation and an increase in LPCAT2 levels (Figure 4A–C).