In accordance with this hypothesis, CUT&RUN analysis showed that there was a marked binding activity of SATB1 promoter region to Twist1 (Fig. 7D); SATB1 mRNA expression was substantially reduced by Twist1 knockdown (Fig. 7E), verified by immunoblot analysis showing that siRNA-mediated knockdown of Twist1 abrogated SATB1 expression in GBM ECs (Fig. 7F). This evidence concerns the gene SATB1 and glioblastoma.