To explore expression signatures of immunosuppressive-like genes across different cell populations within GBM tumors, we performed a single-cell RNA sequencing (scRNA-seq) analysis of genetically engineered murine GBM tumors that were generated by Replication-competent avian sarcoma-leukosis virus long terminal repeat with splice acceptor (RCAS)/N-tva–mediated somatic Pdgfb gene transfer in Ink4a-Arf−/−;Pten−/− neural stem/progenitor cells (Fig. 1A) (2, 10, 11). The gene discussed is PTEN; the disease is glioblastoma.