Notably, scRNA-seq analysis of mouse GBM tumors revealed that Twist1 was highly and exclusively expressed in the ECs-2 cluster, in contrast to a more universal pattern of Zeb1/2 expression in different cell clusters, or a much weaker expression of Snai1, Snai2, or Twist2 in this ECs-2 cluster (Fig. 1I). The gene discussed is TWIST1; the disease is glioblastoma.