For in vivo analysis, we crossed Twist1fl/fl mice with Cdh5-Cre mice to generate an EC-specific Twist1 knockout mouse line, Cdh5-CreERT2;Twist1fl/fl, and used this line to investigate the role of endothelial Twist1 for tumor immunity regulation (Fig. 2D). The gene discussed is CDH5; the disease is neoplasm.