Moreover, we further found that the expression levels of TLR4, NF‐κb (p65), NLPR3, caspase‐1, IL‐1β and IL‐18 in IC/BPS mice were significantly inhibited after intrathecal injection of miR‐9 enriched EVs, suggesting that exosomal miR‐9 derived from MSCs suppress the activity of NLRP3 inflammasome, might by inhibiting the TLR4/NF‐κb signal pathway in SDH of IC mice. This evidence concerns the gene IL1B and Bartsocas-Papas syndrome 1.