Taken together, this study demonstrated that EVs derived from miR‐9‐enriched MSCs can freely cross the blood‐brain barrier into the spinal cord tissue, and then target TLR4 receptors on the activated spinal cord glial cell membrane of IC/BPS model to inhibit the activation of NLRP3 inflammatorome and release of inflammatory factors, which can reduce neuron stimulation and restore neuron firing threshold, so as to desensitize neurons and achieve the purpose of treating bladder pain in IC/BPS patients. This evidence concerns the gene NLRP3 and Bartsocas-Papas syndrome 1.